Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 1. Corneal graft survival curves of BALB/c mice correlated with the endostatin kinetics of the grafts. (A) Corneal graft survival curves (n = 12). All syngeneic corneal grafts survived (BALB/c [H-2d] to BALB/c [H-2d]), whereas 75% of allografts (C57BL/6 [H-2b] to BALB/c [H-2d]) were rejected between POD20 and POD60. (B) Kinetics of endostatin production. After corneal transplantation, upregulation of endostatin production occurred in both allogeneic and syngeneic transplants. Although syngeneic transplants retained high endostatin levels, endostatin production in allogeneic transplants started to decline after POD10 and was significantly decreased by POD20. *p , 0.05. (C) Kinetics of VEGF production. VEGF expression increased in both groups after transplantation, began decreasing after POD3, and increased again after POD10. Allografts retained higher VEGF production compared with syngeneic grafts (p , 0.05). (D) DiI staining identified a significant number of blood vessels in allogeneic grafts, whereas no vessels were detected in syngeneic grafts at POD40. Original magnification 35.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Transplantation Assay, Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 2. Immunohistochemical analysis of endostatin expression and T cell infiltration. Upper panels, Endostatin staining showed that endostatin production in syngeneic grafts re- mained elevated, and no infiltrating T cells were detected. Lower panels, In allogeneic grafts, endostatin production peaked at POD10; con- currently, T cell recruitment into the allograft started around POD10 and increased thereafter, with concomitant graft rejection. Infiltrating T cells in the allografts surrounded the endo- statin-expressing cells. As the number of T cells increased, the number of endostatin-producing cells decreased. The tissue orientation shows the epithelium at the top and the endothelium at the bottom. Images are representative of three mice/group. Scale bar, 50 mm.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 5. Endostatin suppressed the neovascularization of the corneal allografts. (A) Real-time PCR showed that there was more relative mRNA expression of VEGFR2 in the allografts treated with PBS in comparison with allografts treated with endostatin. (B) Immunohistochemical staining showed that fewer CD31+ cells concentrated in the corneal stroma in the endostatin- treated group compared with the PBS-treated group (n = 3 mice/group). (C) DiI staining of blood vessels demonstrates that PBS-treated allografts had significant staining of vessels in the host and graft (left panel), whereas fewer vessels originating from the limbus were seen in the endostatin-treated group, in which the vessels extended to the graft margin but did not extend beyond it into the grafts (right panel). Original magnification 35.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Comparison, Immunohistochemical staining, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Immunological disruption of antiangiogenic signals by recruited allospecific T cells leads to corneal allograft rejection.

doi: 10.4049/jimmunol.1103216

Figure Lengend Snippet: FIGURE 6. T cell infiltration decreased in endostatin-treated corneal allografts at POD40. (A and B) Real-time PCR analysis showed increased CD4 and CD8 expression in the PBS-treated group compared with the endostatin-treated group. (C and D) Immunostaining of T cells showed that there were more CD4+ and CD8+ T cells infiltrated into the corneal stroma of the PBS-treated group compared with the endostatin-treated group (n = 3 mice/group). (E and F) DAPI nuclear staining of corneal grafts. The stroma of endostatin-treated allografts maintained its structural integrity, whereas PBS-treated allografts had thicker and structurally disorganized stroma. Original magnification 320.

Article Snippet: Recombinant mouse endostatin (R&D Systems) was used as the standard protein.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Immunostaining, Staining

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Endostatin in the medium of cell-seeded, lipoplex-supplemented collagen constructs and control constructs. (a) Mesenchymal-stem-cell (MSC)-seeded type I/III constructs cultured in chondrogenic medium (CM) and nonchondrogenic medium (NCM) (n = 3 for first 6 days of lipoplex-supplemented scaffolds; n = 2 for all others). (b) Modification of the scaffold via crosslinking none, half, or all of the lipoplexes (n = 6), in MSC-seeded type I/III constructs cultured in CM. (c) MSCs cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3). (d) Chondrocytes cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3).

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Control, Cell Culture, Modification

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Micrographs of histological sections of chondrocyte- and MSC-seeded constructs cultured in CM and NCM at standard oxygen. (a) Endostatin immunohistochemistry of MSC-seeded CI scaffold supplemented with 20 μg pEndo after 6 days of culture in NCM (red chromogen [arrows] indicates positive stain). Scale bar, 50 μm, 40 × magnification. (b) Hematoxylin and eosin stain of the interior of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 100 μm, 10 × magnification. (c) Hematoxylin and eosin stain of the surface of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 100 μm, 10 × magnification. (d) Safranin-O staining of glycosaminoglycan (GAG) for MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 200 μm, 10× magnification. (e) Type II collagen immunohistochemistry of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red indicates positive stain). Scale bar, 200 μm, 10 × magnification. (f) Safranin-O staining of GAG for chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 500 μm, 4 × magnification. (g) Type II collagen immunohistochemistry of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (brownish-red chromogen indicates positive stain). Scale bar, 500 μm, 4 × magnification. (h) Masson's trichrome staining of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (blue chromogen indicates collagen, red chromogen indicates cytoplasm, and black chromogen indicates nuclei). Scale bar, 500 μm, 4 × magnification. Color images available online at www.liebertonline.com/ten.

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Cell Culture, Immunohistochemistry, Staining, H&E Stain

Journal: Cell death & disease

Article Title: Intelligent drugs based on notch protein remodeling: a defensive targeting strategy for tumor therapy.

doi: 10.1038/s41419-024-07008-7

Figure Lengend Snippet: Fig. 6 Quantitative analysis of iMSCEndostatin’s ability to deploy and produce endostatin. A Fluorescence intensity at various time points following iMSCEndostatin recognition of tumors. B The endostatin-mCherry released by iMSCEndostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSCEndostatin. D The amount of released endostatin by iMSCEndostatin was quantified using ELISA detection.

Article Snippet: ELISA: MSCs or iMSCEndostatin will be co-cultured with Skov3-GFP for 48 h, and MSCs and iMSCEndostatin cells will be sorted by flow cytometry and cultured in a 24-well plate coated with fibronectin for 24 h. The content of endostatin will be detected using an ELISA detection kit (CSB-E07973h; CUSABIO).

Techniques: Fluorescence, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Effect of Niacin on Inflammation and Angiogenesis in a Murine Model of Ulcerative Colitis

doi: 10.1038/s41598-017-07280-y

Figure Lengend Snippet: Effect of niacin on proangiogenic and antiangiogenic factors in colonic tissues of rats with iodoacetamide-induced colitis. (a) Vascular endothelial growth factor (VEGF), (b) angiostatin, (c) endostatin. Data are expressed as means ± SEM of 8 animals. # P ≤ 0.05 vs. normal control, * P ≤ 0.05 vs. colitis control.

Article Snippet: Rat specific VEGF and endostatin ELISA kits were from Cusabio Biotech Co., Ltd. (Wuhan, Hubei, China).

Techniques: Control